2OZM
Crystal structure of RB69 gp43 in complex with DNA with 5-NITP opposite an abasic site analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.232, 117.492, 126.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.860 |
| R-factor | 0.20977 |
| Rwork | 0.204 |
| R-free | 0.26836 |
| Starting model (for MR) | 1ig9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.144 | 0.374 |
| Number of reflections | 28816 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 94.7 | 71.6 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 20000,sodium acetate, magnesium acetate, beta-mercaptoethanol, TRIS 7.5, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 20000 | ||
| 10 | 1 | 2 | TRIS 7.5 | ||
| 11 | 1 | 2 | glycerol | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | beta-mercaptoethanol | ||
| 5 | 1 | 1 | TRIS 7.5 | ||
| 6 | 1 | 1 | glycerol | ||
| 7 | 1 | 2 | PEG 20000 | ||
| 8 | 1 | 2 | sodium acetate | ||
| 9 | 1 | 2 | magnesium acetate |
