2OZK
Structure of an N-Terminal Truncated Form of Nendou (NSP15) From SARS-CORONAVIRUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-03-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 |
Unit cell lengths | 98.974, 98.974, 214.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.750 - 2.900 |
R-factor | 0.24624 |
Rwork | 0.243 |
R-free | 0.30068 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h85 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.485 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.750 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.085 | 0.466 |
Number of reflections | 52135 | |
<I/σ(I)> | 14.4 | 1.44 |
Completeness [%] | 98.1 | 89.9 |
Redundancy | 2.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M sodium bromide, 0.1 M sodium acetate, PH 5.5 and 25% PEG 2000MME, VAPOR DIFFUSION, SITTING DROP, temperature 298K |