2OZE
The Crystal structure of Delta protein of pSM19035 from Streptoccocus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 82.968, 82.968, 233.998 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.500 - 1.830 |
| Rwork | 0.192 |
| R-free | 0.21804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bej |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.062 | 0.396 |
| Number of reflections | 43046 | |
| <I/σ(I)> | 11.8 | 3.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.9M Na-Hepes, 2% Ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
