2OZ6

Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	98
Detector technology	CCD
Collection date	2005-01-17
Detector	BRUKER PROTEUM
Spacegroup name	I 41
Unit cell lengths	69.950, 69.950, 147.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.190
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g6n chain A
RMSD bond length	0.009
RMSD bond angle	1.404
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	8735
<I/σ(I)>	8.5	3.79
Completeness [%]	99.8	99.5
Redundancy	5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Sodium malonate, Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5	298	Sodium malonate, Sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K