2OZ3
Crystal structure of L-Rhamnonate dehydratase from Azotobacter vinelandii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97960 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.772, 115.397, 164.113 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.25702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | 0.670 |
| Number of reflections | 412398 | |
| <I/σ(I)> | 3.7 | 0.6 |
| Completeness [%] | 98.1 | 94 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 60% Tacsimate, pH 7.0, 10% Glycerol, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
