2OYF
Crystal Structure of the complex of phospholipase A2 with indole acetic acid at 1.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.90 |
Spacegroup name | P 43 |
Unit cell lengths | 52.310, 52.310, 47.835 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.18863 |
Rwork | 0.188 |
R-free | 0.20756 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sxk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.418 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 40258 | |
<I/σ(I)> | 50.6 | 4.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 300 | 0.2M ammonium sulphate, 30% PEG 4000, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 300K |