2OYA
Crystal structure analysis of the dimeric form of the SRCR domain of mouse MARCO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-10 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.09700 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.087, 36.553, 44.605 |
| Unit cell angles | 116.30, 90.35, 96.41 |
Refinement procedure
| Resolution | 14.912 - 1.770 |
| R-factor | 0.154 |
| Rwork | 0.150 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oy3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.248 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.912 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.037 | 0.074 |
| Total number of observations | 7835 | |
| Number of reflections | 16364 | |
| <I/σ(I)> | 13.8 | 9.1 |
| Completeness [%] | 95.4 | 89.4 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 7% PEG 4000, 0.1M Bis-Tris, 0.1M Lithium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
