2OY5
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.004, 57.602, 67.746 |
| Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.18723 |
| Rwork | 0.185 |
| R-free | 0.22365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.623 |
| Number of reflections | 28857 | |
| <I/σ(I)> | 24.26 | 2.37 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG4000, 8.5% isopropanol, 0.1M HEPES, 15% Glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
