2OY2
Human MMP-8 in complex with peptide IAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.207, 68.533, 78.281 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 38.750 - 1.500 |
| R-factor | 0.1664 |
| Rwork | 0.164 |
| R-free | 0.19237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bzs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.082 | 0.314 |
| Number of reflections | 55607 | |
| <I/σ(I)> | 6.6 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris-HCl, 20% PEG3350, 200mM acetohydroxamic acid, 0.2M MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
