2OY0
Crystal structure of the West Nile virus methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00925 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.229, 66.009, 78.761 |
| Unit cell angles | 112.46, 102.90, 89.80 |
Refinement procedure
| Resolution | 39.120 - 2.800 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.120 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 0.214 |
| Number of reflections | 15957 | |
| <I/σ(I)> | 4.3 | 2.5 |
| Completeness [%] | 0.9 | 0.917 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 13 to 15% PEG4000, 5% isopropanol, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
