2OXZ
Human MMP-12 in complex with two peptides PQG and IAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-05 |
| Detector | KODAK |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.546, 60.378, 54.449 |
| Unit cell angles | 90.00, 115.41, 90.00 |
Refinement procedure
| Resolution | 30.190 - 1.900 |
| R-factor | 0.21406 |
| Rwork | 0.207 |
| R-free | 0.28767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y93 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.860 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.190 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.137 | 0.327 |
| Number of reflections | 11726 | |
| <I/σ(I)> | 4.7 | 2.3 |
| Completeness [%] | 98.1 | 98.1 |
| Redundancy | 5.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris-HCl, 30% PEG6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
