2OXV
Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 116.473, 116.473, 48.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.950 |
| Rwork | 0.202 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ckq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.077 | 0.436 |
| Number of reflections | 27856 | |
| <I/σ(I)> | 25.1 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | cryoprotectant solution is 40mM bis-tris Propane, 16% v/v PEG 400, 15% v/v glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 2 | PEG 400 | ||
| 4 | 1 | 2 | glycerol | ||
| 5 | 1 | 3 | bis-tris Propane |
