2OXU
Uninhibited form of human MMP-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.545, 60.751, 54.264 |
| Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
| Resolution | 19.640 - 1.240 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y93 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 1.300 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.079 | 0.126 |
| Number of reflections | 41969 | |
| <I/σ(I)> | 3.8 | 5.1 |
| Completeness [%] | 97.2 | 84.8 |
| Redundancy | 5.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris-HCl, 30% PEG6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
