2OXS
Crystal Structure of the trypsin complex with benzamidine at high temperature (35 C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.756, 58.484, 67.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.190 - 1.320 |
| R-factor | 0.15539 |
| Rwork | 0.155 |
| R-free | 0.15814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.522 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.320 |
| Number of reflections | 51449 | |
| <I/σ(I)> | 42.2 | 4.5 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 308 | PEG 9000, 2,5mM benzamidine, ammonium sulphate. CaCl2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 308K |
