2OXO
Crystallization and structure determination of the core-binding domain of bacteriophage lambda integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-21 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.693, 46.693, 111.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.070 - 2.000 |
| R-factor | 0.23426 |
| Rwork | 0.228 |
| R-free | 0.28878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.623 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.073 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.035 | 0.174 |
| Number of reflections | 8503 | |
| <I/σ(I)> | 34.2 | 7.9 |
| Completeness [%] | 74.5 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris, 0.2 M lithium sulfate, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
