2OX1
Archaeal Dehydroquinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.363, 96.060, 117.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.330 |
| R-factor | 0.20828 |
| Rwork | 0.205 |
| R-free | 0.27713 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.627 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.040 | 0.293 |
| Number of reflections | 43264 | |
| <I/σ(I)> | 17.7 | 4 |
| Completeness [%] | 96.2 | 93.3 |
| Redundancy | 3.36 | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | 0.2M ammonium acetate 0.1M sodium acetate 30% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
