2OX0

Crystal structure of JMJD2A complexed with histone H3 peptide dimethylated at Lys9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91820
Spacegroup name	P 21 21 2
Unit cell lengths	101.210, 150.004, 57.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.490 - 1.950
R-factor	0.17047
Rwork	0.169
R-free	0.20999
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.139
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.490	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.072	0.589
Number of reflections	64097
<I/σ(I)>	12	2
Completeness [%]	99.6	98.4
Redundancy	3.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1M Citrate, 4mM NiCl2, 20% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K