2OX0
Crystal structure of JMJD2A complexed with histone H3 peptide dimethylated at Lys9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91820 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.210, 150.004, 57.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.490 - 1.950 |
| R-factor | 0.17047 |
| Rwork | 0.169 |
| R-free | 0.20999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.072 | 0.589 |
| Number of reflections | 64097 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M Citrate, 4mM NiCl2, 20% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
