2OWQ
Crystal structure of vaccinia virus uracil-DNA glycosylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.197, 85.197, 139.725 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.270 - 2.400 |
| R-factor | 0.2436 |
| Rwork | 0.241 |
| R-free | 0.29950 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.061 | 0.299 |
| Number of reflections | 23203 | |
| <I/σ(I)> | 11 | 3.4 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 2.68 | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 295 | 1.5 M ammonium sulfate, 12% glycerol, 0.1M Hepes, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
