2OWJ

Structure of an early-microsecond photolyzed state of CO-bjFixLH, dark state

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	287
Detector technology	CCD
Collection date	2002-04-17
Detector	MAR CCD 165 mm
Wavelength(s)	0.8-1.5
Spacegroup name	H 3 2
Unit cell lengths	128.500, 128.500, 58.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.100 - 2.500
R-factor	0.216
Rwork	0.213
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.608
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.100	2.550
High resolution limit [Å]	2.500	2.500
Number of reflections	6352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	4M NaCl, 50mM CAPSO, 1% PEI, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K