2OVW

ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1995-08-23
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	67.930, 78.310, 142.470
Unit cell angles	90.00, 96.98, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.209 *
Rwork	0.209
R-free	0.28000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE (2 MOLECULES IN ASYMMETRIC UNIT)
RMSD bond length	0.013
RMSD bond angle	0.037
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.077	0.270
Number of reflections	57194
<I/σ(I)>	9.6	2.29
Completeness [%]	86.6	54 *
Redundancy	1.9	1.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4 *	16 *	22 % PEG 8K, 0.2 M MAGNESIUM CHLORIDE, PH 6.5 FOR 0.1 M MOPS. METHOD: HANGING DROP VAPOR DIFFUSION THE NATIVE CRYSTALS WERE SOAKED FOR 1 HOUR IN STABILIZING SOLUTION CONTAINING 20 MM OF CELLOBIOSE., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20-26 (%(w/v))
2	1	reservoir		0.15-0.3 (M)
3	1	reservoir	MOPS	0.1 (M)	pH6.4