2OVH
Progesterone Receptor with Bound Asoprisnil and a Peptide from the Co-Repressor SMRT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.475, 87.475, 90.599 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PR LBD from 1A28 with residues 900-933 removed |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.136 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.084 | 0.038 | 0.415 |
| Number of reflections | 26720 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 97.0 | 98.7 | 76.6 |
| Redundancy | 6.5 | 7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris, 0.4M NaCl, 10% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
