2OVF
Crystal Structure of StaL-PAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 87.392, 87.392, 169.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.920 - 2.950 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ov8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.614 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
| Rmerge | 0.048 | 0.025 | 0.532 |
| Number of reflections | 8661 | ||
| <I/σ(I)> | 17.4 | ||
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 11.2 | 9.8 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Bis-Tris propane pH6.5,0.2M NaI, 18% PEG 3350, 10mM PAP, 5mM desulfo-A47934, vapor diffusion, hanging drop, temperature 295K |
