2OVC
Crystal structure of a coiled-coil tetramerization domain from Kv7.4 channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.116 |
Spacegroup name | I 4 |
Unit cell lengths | 33.185, 33.185, 55.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.460 - 2.070 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.586 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.460 | 50.000 | 2.140 |
High resolution limit [Å] | 2.070 | 4.460 | 2.070 |
Rmerge | 0.053 | 0.043 | 0.096 |
Number of reflections | 1841 | ||
<I/σ(I)> | 15.3 | ||
Completeness [%] | 99.6 | 95.9 | 100 |
Redundancy | 7.3 | 7.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 45% MPD, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, hanging drop, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 45% MPD, 0.2M ammonium acetate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, hanging drop, temperature 289K |