2OVB

Crystal Structure of StaL-sulfate complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 61 2 2
Unit cell lengths	88.733, 88.733, 169.691
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.920 - 2.610
R-factor	0.23
Rwork	0.228
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ov8
RMSD bond length	0.010
RMSD bond angle	1.193
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.610	5.620	2.610
Rmerge	0.051	0.038	0.325
Number of reflections	12609
<I/σ(I)>	22.4
Completeness [%]	99.3	99.6	93.4
Redundancy	10.5	9.7	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1M Bis-Tris pH5.5,0.2M Ammonium Sulfate, 22% PEG 3350, vapor diffusion, hanging drop, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.1M Bis-Tris pH5.5,0.2M Ammonium Sulfate, 22% PEG 3350, vapor diffusion, hanging drop, temperature 295K