2OUO
Crystal Structure of the Bromo domain 2 in human Bromodomain Containing Protein 4 (BRD4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-08 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.032, 73.698, 33.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x0j |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.531 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.720 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.065 | 0.618 |
| Number of reflections | 15125 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M MIB, 30% PEG 1000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
