2OUM
The first domain of L1 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-19 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.700, 45.320, 37.890 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.550 |
| R-factor | 0.18147 |
| Rwork | 0.177 |
| R-free | 0.26973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The first domain of L1 from Thermus thermophilus |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.160 | 0.431 |
| Number of reflections | 3392 | |
| <I/σ(I)> | 8.8 | 3.5 |
| Completeness [%] | 94.4 | 89.1 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM acetate Na, pH 8.5, VAPOR DIFFUSION, temperature 277K |
