2OTX
Crystal Structure of A N-terminal Fragment of SKAP-HOM Containing both the Helical Dimerization Domain and the PH Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.205, 56.742, 89.115 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.600 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u5g |
| RMSD bond length | 0.050 |
| RMSD bond angle | 3.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.059 | 0.390 |
| Number of reflections | 15738 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 95.5 | 99.3 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 4000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
