2OTU
Crystal structure of Fv polyglutamine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.128, 142.681, 75.306 |
| Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
| Resolution | 41.000 - 1.680 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.046 | 0.258 |
| Number of reflections | 116627 | |
| <I/σ(I)> | 34.3 | 3.4 |
| Completeness [%] | 97.5 | 91.1 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 298 | 0.1 M sodium citrate, 0.2 M NH4Ac, 27.5% PEG 4000, pH 6.0, EVAPORATION, temperature 298K |
