2OTM

Crystal structure of a putative endoribonuclease (so_1960) from shewanella oneidensis mr-1 at 1.85 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-31
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97931, 0.97885
Spacegroup name	C 1 2 1
Unit cell lengths	112.083, 62.908, 78.689
Unit cell angles	90.00, 93.24, 90.00

Refinement procedure

Resolution	29.210 - 1.850
R-factor	0.152
Rwork	0.151
R-free	0.17700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.484
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.210	29.210	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.105	0.052	0.667
Total number of observations		1958	13148
Number of reflections	46806
<I/σ(I)>	5.9	10.8	1.1
Completeness [%]	100.0	97.8	100
Redundancy	3.8	3.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 0.16M Ca(OAc)2, 20.0% Glycerol, 14.4% PEG 8000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K