2OTL
Girodazole bound to the large subunit of Haloarcula marismortui
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.05 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 213.077, 300.598, 575.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.360 - 2.700 |
Rwork | 0.204 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s72 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.106 | 0.876 |
Number of reflections | 497071 | |
<I/σ(I)> | 7.3 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 292 | PEG 6000, KCl, NH4Cl, MGCl2, KAcetate, pH 5.8, vapor diffusion, sitting drop, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
10 | 1 | 2 | MGCl2 | ||
11 | 1 | 2 | KAcetate | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | NH4Cl | ||
4 | 1 | 1 | MGCl2 | ||
5 | 1 | 1 | KAcetate | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 6000 | ||
8 | 1 | 2 | KCl | ||
9 | 1 | 2 | NH4Cl |