2OT8
Karyopherin Beta2/Transportin-hnRNPM NLS Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.197, 155.001, 141.528 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h4m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.068 | 0.650 |
| Number of reflections | 63697 | |
| <I/σ(I)> | 20 | 1.5 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, 2.7 M potassium formate, 10% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
