2OSZ
Structure of Nup58/45 suggests flexible nuclear pore diameter by intermolecular sliding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 92.705, 92.705, 169.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.850 |
Rwork | 0.250 |
R-free | 0.28800 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.950 |
High resolution limit [Å] | 2.850 | 6.090 | 2.850 |
Rmerge | 0.098 | 0.047 | 0.389 |
Number of reflections | 17216 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 95.7 | 89 | 98.1 |
Redundancy | 4.2 | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 100 mM Bis-Tris, 600 mM CaCl2, 2.7% (w/v) Benzamidine-HCl, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |