2OSA
The Rho-GAP domain of human N-chimaerin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 61 |
| Unit cell lengths | 92.490, 92.490, 47.095 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.430 - 1.800 |
| R-factor | 0.18978 |
| Rwork | 0.187 |
| R-free | 0.25131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xa6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.430 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 21064 | |
| <I/σ(I)> | 13.8 | 1.1 |
| Completeness [%] | 98.2 | 90.4 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% Jeffamine ED-2001, 0.1M HEPES pH 7.0, 0.1 M Adenosine-5-triphosphate disodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
