2OS2
Crystal structure of JMJD2A complexed with histone H3 peptide trimethylated at Lys36
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-18 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54180 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.943, 148.886, 56.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.17863 |
Rwork | 0.176 |
R-free | 0.22552 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2oq7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.059 | |
Number of reflections | 38324 | |
<I/σ(I)> | 20 | 1.9 |
Completeness [%] | 98.4 | 93.3 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG 3350, 0.1 M Citrate, 2 mM NiCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |