2ORI
Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (A193V/Q199R/)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-05 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.694, 75.136, 77.523 |
| Unit cell angles | 90.00, 98.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.228 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eu8 |
| Data scaling software | d*TREK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.990 | 19.990 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
| Rmerge | 0.052 | 0.034 | 0.242 |
| Total number of observations | 17233 | 11853 | |
| Number of reflections | 49183 | ||
| <I/σ(I)> | 16.6 | 50.1 | 4.4 |
| Completeness [%] | 94.8 | 99.4 | 84.9 |
| Redundancy | 3.04 | 3.18 | 2.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | CHESS, PEG 1500, CaCl2, pH 9.0, vapor diffusion, hanging drop, temperature 293K |
