2OQY
The crystal structure of muconate cycloisomerase from Oceanobacillus iheyensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97890 |
Spacegroup name | P 1 |
Unit cell lengths | 104.635, 104.640, 104.462 |
Unit cell angles | 109.50, 109.45, 109.47 |
Refinement procedure
Resolution | 24.670 - 2.000 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.067 |
Number of reflections | 220117 |
Completeness [%] | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% PEG 3350, 0.1M HEPES, 0.2M Sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |