2OQT
Structural Genomics, the crystal structure of a putative PTS IIA domain from Streptococcus pyogenes M1 GAS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924, 0.97938 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.224, 112.781, 60.401 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 35.850 - 2.410 |
| R-factor | 0.21024 |
| Rwork | 0.208 |
| R-free | 0.25445 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.850 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.099 | 0.542 |
| Number of reflections | 23714 | |
| <I/σ(I)> | 34.86 | 2.02 |
| Completeness [%] | 90.2 | 52.8 |
| Redundancy | 6.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 20% PEG3350, 0.2M Li2SO4, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
