2OQQ
Crystal structure of HY5 leucine zipper homodimer from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Wavelength(s) | 1.12714, 0.97880, 0.97895, 0.97114 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.344, 24.583, 76.517 |
| Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.243 |
| R-free | 0.29600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.392 |
| Number of reflections | 6080 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 97.7 | 87.8 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 0.1M Bis-Tris pH6.0 0.1M MgCl2 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
