2OQF
Structure of a synthetic, non-natural analogue of RNase A: [N71K(Ade), D83A]RNase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-08-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 37.578, 50.534, 62.863 |
Unit cell angles | 85.26, 74.31, 82.70 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23555 |
Rwork | 0.229 |
R-free | 0.29120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nui |
RMSD bond length | 0.007 |
RMSD bond angle | 1.214 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 19122 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |