2OPT
Crystal Structure of Apo ActR from Streptomyces coelicolor.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-08 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.648, 79.957, 104.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.370 - 2.050 |
R-factor | 0.19767 |
Rwork | 0.195 |
R-free | 0.24655 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.594 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.370 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.055 | 0.543 |
Number of reflections | 34133 | |
<I/σ(I)> | 34.6 | 3.85 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% PEG4000, 91mM sodium acetate, 45mM Bis-Tris, 23mM KCl, 9.1mM EDTA, 4.6mM Tris, 0.45mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |