2OPC
Structure of Melampsora lini avirulence protein, AvrL567-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-22 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.818, 52.379, 70.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.460 - 1.430 |
R-factor | 0.19825 |
Rwork | 0.197 |
R-free | 0.22593 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.617 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.468 |
High resolution limit [Å] | 1.430 | 1.432 |
Rmerge | 0.056 | 0.238 |
Number of reflections | 28149 | |
<I/σ(I)> | 33.7 | 13.95 |
Completeness [%] | 93.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290.05 | 10% PEG 8000, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.05K |