2OP4
Crystal Structure of Quorum-Quenching Antibody 1G9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.925, 72.041, 116.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.400 - 2.850 |
| R-factor | 0.20255 |
| Rwork | 0.200 |
| R-free | 0.26329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ntf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.924 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 11938 | |
| <I/σ(I)> | 26.1 | 2.7 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 10% (w/v) PEG 8000, 0.1M Imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
