2OOQ

Crystal Structure of the Human Receptor Phosphatase PTPRT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-01-07
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54180
Spacegroup name	P 1 21 1
Unit cell lengths	37.186, 86.697, 91.120
Unit cell angles	90.00, 99.89, 90.00

Refinement procedure

Resolution	89.770 - 1.800
R-factor	0.14709
Rwork	0.145
R-free	0.18758
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1RPM 2c7s 1lar 2fh7
RMSD bond length	0.015
RMSD bond angle	1.453
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.770	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.092	0.595
Number of reflections	52802
<I/σ(I)>	16	2.6
Completeness [%]	100.0	100
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M LiCl, 0.1M HEPES, 20.0% PEG 6000, 10.0% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K