2OOG
Crystal structure of glycerophosphoryl diester phosphodiesterase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.312, 183.546, 176.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.2362 |
| Rwork | 0.235 |
| R-free | 0.28044 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ydy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.320 |
| Number of reflections | 124065 | |
| <I/σ(I)> | 8 | 1.8 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 5.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | O.1M HEPES PH 7.5, 25% PEG 3350, 0.2M AMMONIUM SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
