2OO1

Crystal structure of the Bromo domain 2 of human Bromodomain containing protein 3 (BRD3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97910
Spacegroup name	P 21 21 2
Unit cell lengths	103.837, 64.678, 91.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.187
Rwork	0.186
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x0j
RMSD bond length	0.021
RMSD bond angle	1.749
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.094
Number of reflections	69114
<I/σ(I)>	6.6
Completeness [%]	99.7	100
Redundancy	4.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2M NaF, 0.1M BTProp, 20% PEG3350, 10% EtGly, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K