2OMI
Structure of human insulin cocrystallized with protamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.520, 61.840, 86.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.240 |
| R-factor | 0.20276 |
| Rwork | 0.199 |
| R-free | 0.26838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | insulin hexamer R-conformation |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.941 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.107 | 0.286 |
| Number of reflections | 15881 | |
| <I/σ(I)> | 12.1 | 4.1 |
| Completeness [%] | 98.6 | 97.6 |
| Redundancy | 6.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 50mM resorcinol, 400mM NaCl, 1.0mg/ml protamine 30mM phosphate buffer, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
