2OMB
Bence Jones KWR Protein- Immunoglobulin Light Chain Dimer, P3(1)21 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.080 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 153.520, 153.520, 93.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dcl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.020 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 27674 | |
| <I/σ(I)> | 14.7 | 2.3 |
| Completeness [%] | 96.6 | 90.1 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.5 M Ammonium Sulfate, 0.1M Tris/HCl pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
