2OLM
ArfGap domain of HIV-1 Rev binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.883, 57.883, 83.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.480 |
| Rwork | 0.180 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.459 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 3.190 | 1.480 |
| Rmerge | 0.055 | 0.042 | 0.237 |
| Number of reflections | 45446 | 4570 | 4449 |
| <I/σ(I)> | 18.9 | ||
| Completeness [%] | 99.7 | 99.5 | 97.3 |
| Redundancy | 7.2 | 7.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.27M Ammonium phosphate, 0.09M BTP, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
