2OL8
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.356, 53.226, 65.195 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.23443 |
| Rwork | 0.232 |
| R-free | 0.28612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.335 |
| Number of reflections | 20849 | |
| <I/σ(I)> | 27.43 | 4.17 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.45 | 293 | 38% PEG400, and 100mM Imidazole, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
