2OL5
Crystal Structure of a protease synthase and sporulation negative regulatory protein PAI 2 from Bacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-20 |
Detector | SBC-3 |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.601, 77.949, 89.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.810 - 2.500 |
R-factor | 0.21911 |
Rwork | 0.213 |
R-free | 0.27551 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.764 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.067 | 0.341 |
Number of reflections | 34879 | |
<I/σ(I)> | 8.7 | 2.27 |
Completeness [%] | 81.7 | 36.7 |
Redundancy | 4.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1M Ammonium acetate, 0.1M Bis-Tris pH 6.0, 17% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |